The Art of Protein Purification
نویسنده
چکیده
Describing, in words, the details of protein purification to a relative novice in the field is not unlike explaining on paper the steps required to turn a set of colored oils into a beautiful pastoral scene on sheet of stretched canvas. Playing the oboe in a sophisticated metropolitan orchestra or performing a solo aria in a Gilbert & Sullivan operetta are accepted artistic endeavors that command great mastery of technique. Each of these art forms requires years of experience and endless experimentation and refinement of technique. Protein purification is no different. It is an art form. Like all other art forms, perfecting the art of protein purification requires a long apprenticeship. But, like all other art forms, protein purification is aesthetically rewarding to the practitioner. Every day brings new challenges, new insights, new hurdles, and new successes. Art is a process, not a destination. Protein purification fits the same definition.
منابع مشابه
Proteins Separation and Purification Methods with Focus on Chromatography: a Review Study
Before describing the structure and mechanism of action of a protein, it must first be subject to purification procedure. Protein purification is a set of processes in which one or a small number of proteins are purified from a complex compound that may be a complete cell, tissue, or organism. Understanding the functions, structural properties, and interactions of the protein are directly re...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملRapid purification of HU protein from Halobacillus karajensis
The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملExpression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi
To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...
متن کاملEvaluation and comparison of affinity chromatography and precipitation– based methods on purification of recombinant streptokinase
Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...
متن کامل